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cpd  (Sino Biological)


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    Structured Review

    Sino Biological cpd
    Cpd, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpd+1+his+protein/Canine+PD-1+Protein+(AVI+%26+His+Tag)%2C+Biotinylated/pm38047502-208-28-31
    Average 94 stars, based on 1 article reviews
    cpd - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Development and characterization of mouse anti-canine PD-L1 monoclonal antibodies and their expression in canine tumors by immunohistochemistry in vitro .
    Article Snippet: .. Canine PD-1/canine PD-L1 neutralizing activity by ELISA Culture supernatant or purified monoclonal antibody (60 μl/well) and cPD-L1 hu Fc (3 μg/ml, 60 μl/well) were co-incubated with shaking (600 rpm) in a 96-well V-shaped plate at 37 °C for 30 min. A 100 μl of co-incubated reaction was added to an ELISA plate coated with cPD-1-His protein (70109-D08H, Sino Biological Inc., Beijing, China) at 100 ng/well, followed by incubation with goat anti-human IgG Fcγ-HRP antibody dilution at 1:5000 in PBST, and finally with the SIGMAFAST OPD substrate solution (Sigma Aldrich, Saint Louis, MO, USA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Development and characterization of mouse anti-canine PD-L1 monoclonal antibodies and their expression in canine tumors by immunohistochemistry in vitro .
    Article Snippet: .. Canine PD-1/canine PD-L1 neutralizing activity by ELISA Culture supernatant or purified monoclonal antibody (60 μl/well) and cPD-L1 hu Fc (3 μg/ml, 60 μl/well) were co-incubated with shaking (600 rpm) in a 96-well V-shaped plate at 37 °C for 30 min. A 100 μl of co-incubated reaction was added to an ELISA plate coated with cPD-1-His protein (70109-D08H, Sino Biological Inc., Beijing, China) at 100 ng/well, followed by incubation with goat anti-human IgG Fcγ-HRP antibody dilution at 1:5000 in PBST, and finally with the SIGMAFAST OPD substrate solution (Sigma Aldrich, Saint Louis, MO, USA). ..

    Article Title: Development and characterization of mouse anti-canine PD-L1 monoclonal antibodies and their expression in canine tumors by immunohistochemistry in vitro
    Article Snippet: The absorbance was measured at 492 nm using a Cytation 5-cell imaging multi-mode reader (BioTek, Winooski, VT, USA). .. Culture supernatant or purified monoclonal antibody (60 μl/well) and cPD-L1 hu Fc (3 μg/ml, 60 μl/well) were co-incubated with shaking (600 rpm) in a 96-well V-shaped plate at 37 °C for 30 min. A 100 μl of co-incubated reaction was added to an ELISA plate coated with cPD-1-His protein (70109-D08H, Sino Biological Inc., Beijing, China) at 100 ng/well, followed by incubation with goat anti-human IgG Fcγ-HRP antibody dilution at 1:5000 in PBST, and finally with the SIGMA FAST OPD substrate solution (Sigma Aldrich, Saint Louis, MO, USA). ..

    Purification:

    Article Title: Development and characterization of mouse anti-canine PD-L1 monoclonal antibodies and their expression in canine tumors by immunohistochemistry in vitro .
    Article Snippet: .. Canine PD-1/canine PD-L1 neutralizing activity by ELISA Culture supernatant or purified monoclonal antibody (60 μl/well) and cPD-L1 hu Fc (3 μg/ml, 60 μl/well) were co-incubated with shaking (600 rpm) in a 96-well V-shaped plate at 37 °C for 30 min. A 100 μl of co-incubated reaction was added to an ELISA plate coated with cPD-1-His protein (70109-D08H, Sino Biological Inc., Beijing, China) at 100 ng/well, followed by incubation with goat anti-human IgG Fcγ-HRP antibody dilution at 1:5000 in PBST, and finally with the SIGMAFAST OPD substrate solution (Sigma Aldrich, Saint Louis, MO, USA). ..

    Article Title: Development and characterization of mouse anti-canine PD-L1 monoclonal antibodies and their expression in canine tumors by immunohistochemistry in vitro
    Article Snippet: The absorbance was measured at 492 nm using a Cytation 5-cell imaging multi-mode reader (BioTek, Winooski, VT, USA). .. Culture supernatant or purified monoclonal antibody (60 μl/well) and cPD-L1 hu Fc (3 μg/ml, 60 μl/well) were co-incubated with shaking (600 rpm) in a 96-well V-shaped plate at 37 °C for 30 min. A 100 μl of co-incubated reaction was added to an ELISA plate coated with cPD-1-His protein (70109-D08H, Sino Biological Inc., Beijing, China) at 100 ng/well, followed by incubation with goat anti-human IgG Fcγ-HRP antibody dilution at 1:5000 in PBST, and finally with the SIGMA FAST OPD substrate solution (Sigma Aldrich, Saint Louis, MO, USA). ..

    Incubation:

    Article Title: Development and characterization of mouse anti-canine PD-L1 monoclonal antibodies and their expression in canine tumors by immunohistochemistry in vitro .
    Article Snippet: .. Canine PD-1/canine PD-L1 neutralizing activity by ELISA Culture supernatant or purified monoclonal antibody (60 μl/well) and cPD-L1 hu Fc (3 μg/ml, 60 μl/well) were co-incubated with shaking (600 rpm) in a 96-well V-shaped plate at 37 °C for 30 min. A 100 μl of co-incubated reaction was added to an ELISA plate coated with cPD-1-His protein (70109-D08H, Sino Biological Inc., Beijing, China) at 100 ng/well, followed by incubation with goat anti-human IgG Fcγ-HRP antibody dilution at 1:5000 in PBST, and finally with the SIGMAFAST OPD substrate solution (Sigma Aldrich, Saint Louis, MO, USA). ..

    Article Title: Development and characterization of mouse anti-canine PD-L1 monoclonal antibodies and their expression in canine tumors by immunohistochemistry in vitro
    Article Snippet: The absorbance was measured at 492 nm using a Cytation 5-cell imaging multi-mode reader (BioTek, Winooski, VT, USA). .. Culture supernatant or purified monoclonal antibody (60 μl/well) and cPD-L1 hu Fc (3 μg/ml, 60 μl/well) were co-incubated with shaking (600 rpm) in a 96-well V-shaped plate at 37 °C for 30 min. A 100 μl of co-incubated reaction was added to an ELISA plate coated with cPD-1-His protein (70109-D08H, Sino Biological Inc., Beijing, China) at 100 ng/well, followed by incubation with goat anti-human IgG Fcγ-HRP antibody dilution at 1:5000 in PBST, and finally with the SIGMA FAST OPD substrate solution (Sigma Aldrich, Saint Louis, MO, USA). ..



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